cell culture a204  (ATCC)

Journal: BMC cancer

Article Title: Histone deacetylase inhibitor panobinostat induces antitumor activity in epithelioid sarcoma and rhabdoid tumor by growth factor receptor modulation.

doi: 10.1186/s12885-021-08579-w

Figure Lengend Snippet: Fig. 2 Rhabdoid tumor is more sensitive to in vitro inhibition of growth factor receptors than epithelioid sarcoma. A Half-maximal inhibitory concentration (IC50) after 72 h, based on confluence (IncuCyte®) or MTS assay (*) in N ≥3 biological replicates. B EGFR, FGFR1 and FGFR2 mRNA expression levels of A204, VAESBJ, and GRU1 cells were determined by RT-qPCR. The scatter plot shows measurements of N ≥3 biological replicates in 3 technical replicates, normalized on GAPDH expression (dCT (cycle threshold) values; median ± IQR ). C Cell proliferation in response to EGF and FBS10% in serum-starved cells by IncuCyte®, graph represents means ± SEM of N ≥3 biological replicates. D Effects on AKT and ERK activity after treatment with pazopanib (left panel) and regorafenib (right panel) after 5, 30 and 120 min, assessed by Western blot (ratio candidate protein/β-ACTIN, related to expression of control)

Article Snippet: Cell lines and cell culture Rhabdoid tumor cell line A204 was purchased from Leibniz Institute DSMZ (German Collection of Microrganisms and Cell Cultures), epithelioid sarcoma cell line VAESBJ from ATCC® (American Type Culture Collection).

Techniques: In Vitro, Inhibition, Concentration Assay, MTS Assay, Expressing, Quantitative RT-PCR, Activity Assay, Western Blot, Control

Journal: BMC cancer

Article Title: Histone deacetylase inhibitor panobinostat induces antitumor activity in epithelioid sarcoma and rhabdoid tumor by growth factor receptor modulation.

doi: 10.1186/s12885-021-08579-w

Figure Lengend Snippet: Fig. 3 Regorafenib and panobinostat induce tumor growth inhibition in vivo. Mice xenografted with A, VAESBJ tumors and A204 tumors were treated daily with 30 mg/kg regorafenib p.o.; graph represents means ± SEM of N = 8 to 9 tumors per group. Statistical analysis in both experiments was performed using the nonparametric Mann-Whitney test; p < 0.001 each. B Animals bearing VAESBJ tumors were treated with 8 and 12 mg/kg panobinostat i.p. three times per week; graph represents means ± SEM of N = 11 to 16 tumors per group. Statistical analysis was performed using the nonparametric Kruskal-Wallis test; p < 0.05 for 8 mg/kg and p < 0.0005 for 12 mg/kg. C ERK and AKT activation in tumors harvested after 24 h of a single dose of 8 and 12 mg/kg panobinostat i.p. are shown by Western blot

Article Snippet: Cell lines and cell culture Rhabdoid tumor cell line A204 was purchased from Leibniz Institute DSMZ (German Collection of Microrganisms and Cell Cultures), epithelioid sarcoma cell line VAESBJ from ATCC® (American Type Culture Collection).

Techniques: Inhibition, In Vivo, MANN-WHITNEY, Activation Assay, Western Blot